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Restriction fragment length polymorphism (RFLP) analysis was developed for genetic ty ping of Polish strains of bovine viral diarrhoea virus (BVDV). The method was applied using 60 BVDV isolates, which included BVDV genotype 1, subtypes a, b, d, e, f, and g, and genotype 2a. RT-PCR products of the 5'untranslated region (5'UTR) were digested using three enzymes. Restriction patterns classified the...
The aim of the studx was to evaluate the influence of sildenafil citrate administrated intravaginaly on the blood flow in the bovine uterus during dioestrus. Uterine blood flow was examined in six healthy adult cows. Sildenafil was administrated intravaginaly to each cow between the 6th and 8th d of the ovarian cycle, in the form of vaginal suppositories containing 100 mg of active substance at a...
The effect of dietary linseed and lucerne supplementation on the oxidative stability of ostrich meat expressed by changes in concentrations of malondialdehyde (MDA) and glutathione (GSH), and in activity of superoxide dismutase (SOD), was studied. The feeding regimens were as follows: C - control group, L - 4% supplement of linseed, L-L45, L-L55, L-L65, and L-L75 - 4% supplement of linseed and supplement...
The aim of the studies was to develop an alternative method which could overcome the lack of sampling to improve the efficiencx of control efforts for bovine endemic fluorosis. The spatial distribution characteristics of the disease were analysed and a prediction model for the estimation of fluorosis distribution in some districts in northwest Liaoning province in China was established. The model...
The aim of the study was to determine the therapeutic efficacy of simultaneous administration of GnRH and PGF2α in dain cows with ovarian cysts. Ovarian cyst-affected dairy cows were divided into two experimental groups: 54 cows treated with GnRH and PGF2α, and 42 cows treated with GnRH alone, whereas 22 untreated cows served as the control group. Clinical response and reproductive performance were...
A detailed study on histopathological lesions induced by two C. psittaci outer membrane protein A (ompA) genotype B strains (10/423 and 10/525) and one genotype D strain (10/298) in experimentally infected (aerosol) specific pathogen free (SPF) chickens was performed. The strains were derived from Belgian and French commercially raised broilers with pneumonia. Both genotxpe B and D strains induced...
The effect of inflammatory bowel disease (IBD) on the density of galanin - immunoreactive (GAL-IR) nerve fibers was determined in the mucosa of canine duodenum, jejunum, and descending colon. Fiber densitx was evaluated by a single immunofluorescence method in biopsy specimens obtained from healthy dogs and patients with variable severity of the disease. The densit) of GAL-IR nerve fibers was determined...
The purpose of the study was to define transient changes in the concentration of inflammatory biomarkers and cartilage biomarkers in the synovial fluid of joints following experimentally induced acute equine synovitis. Acute synovitis was induced in eight skeletal!) mature mares by a sterile intra-articular injection of 1 mL of phosphate-buffered saline (PBS) containing 0.5 ng of lipopolysaccharide...
The objective of the study was to determine the degree of municipal wastewater contamination with intestinal parasite eggs of the genera Ascaris, Toxocara, and Trichuris at individual stages of treatment, and indication of potentially weak points in the hygienisation of sewage sludge. The study was conducted in 17 municipal mechanical-biological wastewater treatment plants which, to a slight degree,...
The purpose of the study was to investigate the clinical, biochemical, and cardiovascular effects of intrathecal (IT) administration of ketamine HCl in calves. The study was performed on seven Simmental and three Montofon calves, 1.70± 1.16 weeks old, weighing approximately 37 kg, undergoing surgical procedures including femur fracture repair (one case), atresia anus (five cases), prolapsed rectum...
Fenpropathrin (FEN) was administered intraperitoneally in doses of 2.38 mg/kg, 5.9 5mg/kg, or 11.9 mg/kg b.w. to mice for 28 consecutive days. FEN did not significantly affect the activity of alanine transaminase (ALT) in the sera. Superoxide dismutase and glutathione peroxidase activities were significantly elevated in the liver after a 28-d exposure to moderate or highest doses of the pesticide...
The study was conducted on ventricular and atrial wall preparations from 11 dogs with clinically diagnosed dilated cardiomyopathy. After fixation, the specimens were stained with haematoxylin and eosin and Masson-Goldner trichrome technique. Parenchymal changes (fibrosis and fatty infiltration), vascular changes (congestion and coronan vessel wall hypertrophy), degenerative changes (loss of striation,...
A current profile of antimicrobial resistance and plasmid of 29 Lactococcus garvieae and one Lactococcus lactis strains isolated from rainbow trouts (Oncorhynchus mykiss) from farms throughout Turkey were investigated. All isolates were sensitive to penicillin G (90%), ampicillin (86.7%), florfenicol (83.3%), amoxicillin (80.1%), and tetracycline (73.4%), and resistant to trimethoprim+sulfamethoxazole...
This paper is the first study of the prevalence of leptospirosis in the cattle at slaughter from a rural area of Kazakhstan. Five hundred and seventy three samples of serum, urine, and kidneys from cattle of Alatau, Kazakh white and Auliyekol breed, aged from 2 to 5 years (unknown vaccination status), from the province of Almaty in the South-Eastern region were collected during four years (March 2010...
Immunohistochemical profiles of the most common canine testicular tumours, including the Leydig cell tumours, seminomas, and Sertoli cell tumours were analysed, and the results were compared with those obtained in the corresponding txpes of human testicular neoplasms. The expressions of vimentin, von Willebrand factor (FVIII), chromogranin A, synaptophysin, and MCM3 were quantified. In the case of...
The aim of the study was to determine the impact of steroidal medications on the structure and mechanical properties of supporting tissues of sheep under experimentally-induced osteoporosis. A total of 21 sheep were used, divided into three groups: a negative control (KN) (n = 3), a positive control (KP) (n = 3) with ovariectomy, and a steroidal group (KS) (n = 15) with ovariectomy and glucocorticosteroids...
The aim of this studx was to evaluate the preventive effectiveness of dry cow therapy based on antibiotic, internal teat sealant, and α-tocopherol administered separately or in various combinations at dry ing-off. The study was performed on 322 uninfected quarters of 95 cows originating from three dairy herds. The new intramammary infection rates after calving were measured to evaluate the effectiveness...
The paper presents mycological studies conducted jointly with ornithologists on the epidemiology of mycoses and the taxonomie diversity and prevalence of fungi that colonise the selected onthocenoses in health) , wild migratory birds. Aquatic ecosystem populations of healthy birds include a percentage of carriers of potential zoo- and anthropopathogens, and this study's purpose was to determine the...
We investigated whether dairy beef cattle raised in Algeria are Shiga toxin-producing Escherichia coli (STEC) carriers. Stx1 and stx2 genes were analysed in DNA isolated from 200 faecal samples collected from adult dairy cows from 27 randomh selected farms in Blida, North Algeria, after amplification by PCR. Samples from 61 (30.5%) animals out of the 200 were positive and were located in 18 farms...
Thirty clinicalh healthy dogs with poor semen quality were used in the study. Fifteen dogs were supplemented daily with selenium (0.6 mg/kg organic selenium from yeast) and vitamin E (5 mg/kg) per os for 60 d. The control group ( 15 dogs) was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated...
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